Dr. Durgadevi Aphale
Name : Dr. Durgadevi Aphale
Designation : Project Scientist-IIII

Brief Background :

I do have 13 years of Industrial research experience in various research areas including strain development for sustainable fuels, cloning and over-expression, fermentation, animal probiotics, bacteriocins, protein characterization and purification, waste- water treatment, rumen microbiology and molecular biology, bioinformatics, Omics analysis, High Throughput Screening Liquid Handling System, Polyhydroxyalkanoates, Biosurfactant: Human Anti-Cancer Activities.
I have handled the industrial microbial culture collection for more than 7 years, providing proprietary microbial cultures to the domestic and International customers with strong adherence to the guidelines, certifications, and documentation. Currently associated with Agharkar Research Institute and working on development of technology for biofuels production from lignocellulosic feedstocks.

Contact Details :

  • PhD in Microbiology (Dec 2020) – Industrial PhD sponsored by Praj Industries Limited, Pune, India In collaboration with Symbiosis International Deemed University, Pune, India.

    Thesis: “In-vitro characterization of Probiotic and OR Pathogen Induced Variation In the Microbial Community of Rumen”

  • MSc in Microbiology (2008-2010) – Yashwantrao Chavan College of Science Karad, Shivaji University, Kolhapur | Graduated with 77.7%. University Ranker.

    Dissertation Project: “Development of Microbial Enzymes for degradation of Calcium Oxalate Kidney Stones”.

  • BSc in Microbiology (2006-2008) –  Yashwantrao Chavan College of Science Karad, Shivaji University, Kolhapur | Graduated with 78.8%. University Ranker. 

Research areas of Interest

  1. Biofuel production from lignocellulosic feedstocks
  2. Industrial strain development with site directed mutagenesis and advanced molecular biology tools and techniques
  3. Rumen anaerobic microbiology and molecular biology
  4. High Throughput Liquid Handling System
  5. Biosurfactant and Bioplastics
  6. Bioinformatics
  7. Animal probiotics

Current research area

A novel process development for scale up of biofuel production from lignocellulosic feedstocks

  • Rutuja Ankulkar, Sambhaji Chavan, Durgadevi Aphale, Meera Chavan, Yasmin Mirza (2022) Cytotoxicity of di-rhamnolipids produced by Pseudomonas aeruginosa RA5 against human cancerous cell lines. 3 Biotech 12(11):323.
  • Aphale D, Natu A, Laldas S, Sawale S, Kulkarni A, Harsulkar A (2020) Probiotic composition influences the major rumen microbial shifts and fermentation profile as evaluated using quantitative PCR and in vitro RUSITEC system. Biosci Biotech Res Comm 13 (2): 937-946.
  • Aphale D, Laldas S, Kulkarni A (2019) Mapping rumen microbial complexity through ‘- Omics ‘. Archives of Animal and Poultry Sciences 1(2): 001-004.
  • Aphale D, Natu A, Laldas S, Kulkarni A (2019) Administration of Streptococcus bovis isolated from sheep rumen digesta on rumen function and physiology as evaluated in a rumen simulation technique system. Vet World 12(9): 1362-1371.
  • Aphale D, Kulkarni A (2018) Modifications and optimization of manual methods for polymerase chain reaction and 16S rRNA gene sequencing quality community DNA extraction from goat rumen digesta. Vet World 11(7): 990-1000
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